OrangerV1, Main, Exploration, bibRecord, 001295

Identification and Characterization of the Rhizobium sp. Strain GIN611 Glycoside Oxidoreductase Resulting in the Deglycosylation of Ginsenosides

Identifieur interne : 001295 ( Main/Exploration ); précédent : 001294; suivant : 001296

Identification and Characterization of the Rhizobium sp. Strain GIN611 Glycoside Oxidoreductase Resulting in the Deglycosylation of Ginsenosides

Auteurs : Eun-Mi Kim [Corée du Sud] ; Juhan Kim [États-Unis] ; Joo-Hyun Seo [Corée du Sud] ; Jun-Seong Park [Corée du Sud] ; Duck-Hee Kim [Corée du Sud] ; Byung-Gee Kim [Corée du Sud]

Source :

Applied and Environmental Microbiology [ 0099-2240 ] ; 2012.

RBID : PMC:3255640

Abstract

Using enrichment culture, Rhizobium sp. strain GIN611 was isolated as having activity for deglycosylation of a ginsenoside, compound K (CK). The purified heterodimeric protein complex from Rhizobium sp. GIN611 consisted of two subunits with molecular masses of 63.5 kDa and 17.5 kDa. In the genome, the coding sequence for the small subunit was located right after the sequence for the large subunit, with one nucleotide overlapping. The large subunit showed CK oxidation activity, and the deglycosylation of compound K was performed via oxidation of ginsenoside glucose by glycoside oxidoreductase. Coexpression of the small subunit helped soluble expression of the large subunit in recombinant Escherichia coli. The purified large subunit also showed oxidation activity against other ginsenoside compounds, such as Rb1, Rb2, Rb3, Rc, F2, CK, Rh2, Re, F1, and the isoflavone daidzin, but at a much lower rate. When oxidized CK was extracted and incubated in phosphate buffer with or without enzyme, (S)-protopanaxadiol [PPD(S)] was detected in both cases, which suggests that deglycosylation of oxidized glucose is spontaneous.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3255640

DOI: 10.1128/AEM.06404-11
PubMed: 22020506
PubMed Central: 3255640

Affiliations:

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification and Characterization of the <named-content content-type="genus-species">Rhizobium</named-content>
 sp. Strain GIN611 Glycoside Oxidoreductase Resulting in the Deglycosylation of Ginsenosides</title>
<author><name sortKey="Kim, Eun Mi" sort="Kim, Eun Mi" uniqKey="Kim E" first="Eun-Mi" last="Kim">Eun-Mi Kim</name>
<affiliation wicri:level="4"><nlm:aff id="aff1">School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>School of Chemical and Biological Engineering, Seoul National University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
</placeName>
<orgName type="university">Université nationale de Séoul</orgName>
</affiliation>
</author>
<author><name sortKey="Kim, Juhan" sort="Kim, Juhan" uniqKey="Kim J" first="Juhan" last="Kim">Juhan Kim</name>
<affiliation wicri:level="2"><nlm:aff id="aff2">Cooperative Institute for Research in Environmental Sciences, University of Colorado, Boulder, Colorado, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Cooperative Institute for Research in Environmental Sciences, University of Colorado, Boulder, Colorado</wicri:regionArea>
<placeName><region type="state">Colorado</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Seo, Joo Hyun" sort="Seo, Joo Hyun" uniqKey="Seo J" first="Joo-Hyun" last="Seo">Joo-Hyun Seo</name>
<affiliation wicri:level="4"><nlm:aff id="aff1">School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>School of Chemical and Biological Engineering, Seoul National University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
</placeName>
<orgName type="university">Université nationale de Séoul</orgName>
</affiliation>
</author>
<author><name sortKey="Park, Jun Seong" sort="Park, Jun Seong" uniqKey="Park J" first="Jun-Seong" last="Park">Jun-Seong Park</name>
<affiliation wicri:level="1"><nlm:aff id="aff3">R & D Center, Amore-Pacific Corporation, Yong-In, Kyounggi-do, Republic of Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>R & D Center, Amore-Pacific Corporation, Yong-In, Kyounggi-do</wicri:regionArea>
<wicri:noRegion>Kyounggi-do</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kim, Duck Hee" sort="Kim, Duck Hee" uniqKey="Kim D" first="Duck-Hee" last="Kim">Duck-Hee Kim</name>
<affiliation wicri:level="1"><nlm:aff id="aff3">R & D Center, Amore-Pacific Corporation, Yong-In, Kyounggi-do, Republic of Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>R & D Center, Amore-Pacific Corporation, Yong-In, Kyounggi-do</wicri:regionArea>
<wicri:noRegion>Kyounggi-do</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kim, Byung Gee" sort="Kim, Byung Gee" uniqKey="Kim B" first="Byung-Gee" last="Kim">Byung-Gee Kim</name>
<affiliation wicri:level="4"><nlm:aff id="aff1">School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>School of Chemical and Biological Engineering, Seoul National University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
</placeName>
<orgName type="university">Université nationale de Séoul</orgName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">22020506</idno>
<idno type="pmc">3255640</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3255640</idno>
<idno type="RBID">PMC:3255640</idno>
<idno type="doi">10.1128/AEM.06404-11</idno>
<date when="2012">2012</date>
<idno type="wicri:Area/Pmc/Corpus">000C29</idno>
<idno type="wicri:Area/Pmc/Curation">000C28</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000C90</idno>
<idno type="wicri:Area/Ncbi/Merge">000E34</idno>
<idno type="wicri:Area/Ncbi/Curation">000E34</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000E34</idno>
<idno type="wicri:doubleKey">0099-2240:2012:Kim E:identification:and:characterization</idno>
<idno type="wicri:Area/Main/Merge">001309</idno>
<idno type="wicri:Area/Main/Curation">001295</idno>
<idno type="wicri:Area/Main/Exploration">001295</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Identification and Characterization of the <named-content content-type="genus-species">Rhizobium</named-content>
 sp. Strain GIN611 Glycoside Oxidoreductase Resulting in the Deglycosylation of Ginsenosides</title>
<author><name sortKey="Kim, Eun Mi" sort="Kim, Eun Mi" uniqKey="Kim E" first="Eun-Mi" last="Kim">Eun-Mi Kim</name>
<affiliation wicri:level="4"><nlm:aff id="aff1">School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>School of Chemical and Biological Engineering, Seoul National University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
</placeName>
<orgName type="university">Université nationale de Séoul</orgName>
</affiliation>
</author>
<author><name sortKey="Kim, Juhan" sort="Kim, Juhan" uniqKey="Kim J" first="Juhan" last="Kim">Juhan Kim</name>
<affiliation wicri:level="2"><nlm:aff id="aff2">Cooperative Institute for Research in Environmental Sciences, University of Colorado, Boulder, Colorado, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Cooperative Institute for Research in Environmental Sciences, University of Colorado, Boulder, Colorado</wicri:regionArea>
<placeName><region type="state">Colorado</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Seo, Joo Hyun" sort="Seo, Joo Hyun" uniqKey="Seo J" first="Joo-Hyun" last="Seo">Joo-Hyun Seo</name>
<affiliation wicri:level="4"><nlm:aff id="aff1">School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>School of Chemical and Biological Engineering, Seoul National University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
</placeName>
<orgName type="university">Université nationale de Séoul</orgName>
</affiliation>
</author>
<author><name sortKey="Park, Jun Seong" sort="Park, Jun Seong" uniqKey="Park J" first="Jun-Seong" last="Park">Jun-Seong Park</name>
<affiliation wicri:level="1"><nlm:aff id="aff3">R & D Center, Amore-Pacific Corporation, Yong-In, Kyounggi-do, Republic of Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>R & D Center, Amore-Pacific Corporation, Yong-In, Kyounggi-do</wicri:regionArea>
<wicri:noRegion>Kyounggi-do</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kim, Duck Hee" sort="Kim, Duck Hee" uniqKey="Kim D" first="Duck-Hee" last="Kim">Duck-Hee Kim</name>
<affiliation wicri:level="1"><nlm:aff id="aff3">R & D Center, Amore-Pacific Corporation, Yong-In, Kyounggi-do, Republic of Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>R & D Center, Amore-Pacific Corporation, Yong-In, Kyounggi-do</wicri:regionArea>
<wicri:noRegion>Kyounggi-do</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kim, Byung Gee" sort="Kim, Byung Gee" uniqKey="Kim B" first="Byung-Gee" last="Kim">Byung-Gee Kim</name>
<affiliation wicri:level="4"><nlm:aff id="aff1">School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>School of Chemical and Biological Engineering, Seoul National University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
</placeName>
<orgName type="university">Université nationale de Séoul</orgName>
</affiliation>
</author>
</analytic>
<series><title level="j">Applied and Environmental Microbiology</title>
<idno type="ISSN">0099-2240</idno>
<idno type="eISSN">1098-5336</idno>
<imprint><date when="2012">2012</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Using enrichment culture, <named-content content-type="genus-species">Rhizobium</named-content>
 sp. strain GIN611 was isolated as having activity for deglycosylation of a ginsenoside, compound K (CK). The purified heterodimeric protein complex from <named-content content-type="genus-species">Rhizobium</named-content>
 sp. GIN611 consisted of two subunits with molecular masses of 63.5 kDa and 17.5 kDa. In the genome, the coding sequence for the small subunit was located right after the sequence for the large subunit, with one nucleotide overlapping. The large subunit showed CK oxidation activity, and the deglycosylation of compound K was performed via oxidation of ginsenoside glucose by glycoside oxidoreductase. Coexpression of the small subunit helped soluble expression of the large subunit in recombinant <named-content content-type="genus-species">Escherichia coli</named-content>
. The purified large subunit also showed oxidation activity against other ginsenoside compounds, such as Rb1, Rb2, Rb3, Rc, F2, CK, Rh2, Re, F1, and the isoflavone daidzin, but at a much lower rate. When oxidized CK was extracted and incubated in phosphate buffer with or without enzyme, (<italic>S</italic>
)-protopanaxadiol [PPD(S)] was detected in both cases, which suggests that deglycosylation of oxidized glucose is spontaneous.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>Corée du Sud</li>
<li>États-Unis</li>
</country>
<region><li>Colorado</li>
</region>
<settlement><li>Séoul</li>
</settlement>
<orgName><li>Université nationale de Séoul</li>
</orgName>
</list>
<tree><country name="Corée du Sud"><noRegion><name sortKey="Kim, Eun Mi" sort="Kim, Eun Mi" uniqKey="Kim E" first="Eun-Mi" last="Kim">Eun-Mi Kim</name>
</noRegion>
<name sortKey="Kim, Byung Gee" sort="Kim, Byung Gee" uniqKey="Kim B" first="Byung-Gee" last="Kim">Byung-Gee Kim</name>
<name sortKey="Kim, Duck Hee" sort="Kim, Duck Hee" uniqKey="Kim D" first="Duck-Hee" last="Kim">Duck-Hee Kim</name>
<name sortKey="Park, Jun Seong" sort="Park, Jun Seong" uniqKey="Park J" first="Jun-Seong" last="Park">Jun-Seong Park</name>
<name sortKey="Seo, Joo Hyun" sort="Seo, Joo Hyun" uniqKey="Seo J" first="Joo-Hyun" last="Seo">Joo-Hyun Seo</name>
</country>
<country name="États-Unis"><region name="Colorado"><name sortKey="Kim, Juhan" sort="Kim, Juhan" uniqKey="Kim J" first="Juhan" last="Kim">Juhan Kim</name>
</region>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001295 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001295 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Bois
   |area=    OrangerV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:3255640
   |texte=   Identification and Characterization of the Rhizobium sp. Strain GIN611 Glycoside Oxidoreductase Resulting in the Deglycosylation of Ginsenosides
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:22020506" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a OrangerV1

This area was generated with Dilib version V0.6.25.
Data generation: Sat Dec 3 17:11:04 2016. Site generation: Wed Mar 6 18:18:32 2024

Serveur d'exploration sur l'oranger

Identification and Characterization of the Rhizobium sp. Strain GIN611 Glycoside Oxidoreductase Resulting in the Deglycosylation of Ginsenosides

Identification and Characterization of the Rhizobium sp. Strain GIN611 Glycoside Oxidoreductase Resulting in the Deglycosylation of Ginsenosides

Source :

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur l'oranger
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.